Kingston Collegiate and Vocational Institute (KCVI) was a secondary school in Kingston, Ontario, Canada. Founded in 1792 by Reverend John Stuart based upon a grant for secondary education in the colony of Upper Canada, it moved to its location at 235 Frontenac Street in 1892. It is considered the oldest public secondary school in Ontario and the second oldest in Canada. The site and remaining buildings were purchased by Queen's University in 2021.

KCVI was Kingston's only public secondary school until the opening of Queen Elizabeth Collegiate and Vocational Institute (QECVI) in 1955 and Loyalist Collegiate and Vocational Institute (LCVI) in 1963.

In 2012, KCVI was ranked by the Fraser Institute as the top performing school in the Limestone Board and in the top 10 per cent of public schools in Ontario.

When KCVI closed in December 2020, its student population moved to Kingston Secondary School, a new school constructed on the former QECVI site that was created to replace both KCVI and QECVI as the result of a Program and Accommodation Review Committee decision reached in 2014.