The University of Guelph (U of G) is a comprehensive public research university in Guelph, in Ontario, Canada. It was established in 1964 after the amalgamation of Ontario Agricultural College (1874), the MacDonald Institute (1903), and the Ontario Veterinary College (1922), and has since grown to an institution of over 30,000 students from more than 140 countries (including those at the Humber campus, Ridgetown campus, off-campus degree enrolments, diploma enrolments and part-time students) and employs 823 full-time faculty (academic staff) as of 2025. It offers 94 undergraduate degrees, 48 graduate programs, and 6 associate degrees in many different disciplines.

The university conducts a significant degree of research and offers a wide range of undergraduate, graduate, and professional degrees. According to the Journal of Hospitality & Tourism Research, the university's Hospitality and Tourism Management program has Canada's highest research index.

The faculty at the University of Guelph hold 23 Canada Research Chair positions in the research areas of natural sciences, engineering, health sciences and social sciences. Academic achievements include the first scientific validation of water on Mars, Alpha Particle X-ray Spectrometer (APXS) on board the Curiosity rover, and the Barcode of Life project for species identification.