The University of Manitoba (U of M, UManitoba, or UM) is a public research university in Winnipeg, Manitoba, Canada. Founded in 1877, it is the first university of Western Canada. Both by total student enrolment and campus area, the University of Manitoba is the largest university in the province of Manitoba. Its main campus is located in the Fort Garry neighbourhood of Winnipeg, with other campuses throughout the city: the Bannatyne Campus, the James W. Burns Executive Education Centre, the William Norrie Centre, and the French-language affiliate, Université de Saint-Boniface in the Saint Boniface ward.

Research at the university contributed to the creation of canola oil in the 1970s. Likewise, University of Manitoba alumni include Nobel Prize recipients, Academy Award winners, Order of Merit recipients, and Olympic medalists. As of 2019, there have been 99 Rhodes Scholarship recipients from the University of Manitoba, more than that of any other university in western Canada.

The University of Manitoba is a member of the U15 group of research-intensive universities in Canada and of Universities Canada, while its global affiliations include the International Association of Universities and the Association of Commonwealth Universities.

The Manitoba Bisons compete in U Sports and Canada West Universities Athletic Association (CWUAA).