The University of Guayaquil (Spanish: Universidad de Guayaquil), known colloquially as the Estatal (i.e., "the State [University]"), is a public university in Guayaquil, Guayas Province, Ecuador.

The University of Guayaquil was founded on December 1, 1867, when the University Board of Guayas was formally established with the authority to award professional degrees in the coastal region of Ecuador. It is the oldest university in the city and holds the largest student body in Ecuador, with over 70,000 enrolled students. The institution operates several university extensions throughout the country.