Cathedral Seminary House of Formation was a Catholic seminary college in the Douglaston, Queens section of New York City. It was founded in 1914 as a minor seminary for the Diocese of Brooklyn, eventually growing to two New York City campuses in Brooklyn and Queens. In 1967, the college programs were detached from the high school program and relocated to Douglaston as a four-year college.

Cathedral College closed in 1987. Today the Cathedral Seminary House of Formation operates on part of the original college campus. The house of formation shut down its seminary program in 2023, but continues to host graduate level programs for diaconate formation and lay faithful.