Mount Holyoke College is a private women's liberal arts college in South Hadley, Massachusetts, United States. It is the oldest member of the historic Seven Sisters colleges, a group of historically women’s colleges in the Northeastern United States. The college was founded in 1837 as the Mount Holyoke Female Seminary by Mary Lyon, a pioneer in education for women. Mount Holyoke is part of the Five College Consortium in Western Massachusetts.

Undergraduate admissions are restricted to female, transgender, and nonbinary students. In 2014, it became the first member of the Seven Sisters (not counting the coeducational Vassar College) to introduce an admissions policy that was inclusive of transgender students. Graduate programs are open to applicants regardless of gender.

The college's 800-acre (3.2 km²) campus includes the Mount Holyoke College Art Museum, the John Payson Williston Observatory, and a botanic garden. The college awards the Glascock Prize annually.