Hamline University ( HAM-lin) is a private university in Saint Paul, Minnesota, United States. Founded in 1854, Hamline is the second oldest university in Minnesota, the first coeducational university in the state, and is one of five Associated Colleges of the Twin Cities. The university is named after Bishop Leonidas Lent Hamline of the United Methodist Church. As of 2017, Hamline had 2,117 undergraduate students and 1,668 graduate students.

In 2022, the university attracted widespread criticism after firing an adjunct professor for showing paintings of the Islamic prophet Muhammad in a class on the history of Islamic art.