Maryville High School is the public high school for Maryville, Missouri. It is the only institution in the United States to have the Spoofhound for a mascot. It is a Missouri State High School Activities Association Class III school. The present high school building on the southwest side of Maryville opened in the 1965–66 school year.

The school is officially Maryville R-II High School. The R-II refers to the 1959 consolidation when 23 school districts voted to reorganize as one district. The reorganization involved the main Maryville school along with 22 rural districts that had one-room schoolhouses. Several other communities in Nodaway County voted in the same election (e.g., R-I, R-II, R-III, etc.) and Maryville was the R-II district in that consolidation.