Middleton High School is a public secondary school located in the city of Middleton, Wisconsin. It was established in 1879. Part of the Middleton-Cross Plains School District, the school serves more than 2,000 students in grades 9 to 12 from the Middleton and Cross Plains areas. Middleton High School's mascot is the cardinal. The school colors are white and cardinal red. Its athletic teams play at the WIAA Division 1 level in the WIAA Big Eight Conference. Middleton High School is often praised for having highly rated STEM and athletics programs.

In 2021, in a report by U.S. News & World Report, Middleton High School was named as the best high school in Dane County and it was also named best high school out of around 540 in the state of Wisconsin.