De La Salle University (Filipino: Pamantasang De La Salle), also referred to as DLSU, De La Salle or La Salle, is a private Catholic research university run by the Institute of the Brothers of the Christian Schools with main campus in Taft Avenue, Malate, Manila, Philippines. It was established by the Christian Brothers in 1911 as De La Salle College (DLSC) in Nozaleda Street, Paco, Manila with Blimond Pierre Eilenbecker, FSC serving as director, and is the first De La Salle school in the Philippines. The college was granted university status on February 19, 1975, and is the oldest constituent of De La Salle Philippines (DLSP), a network of 16 educational institutions, established in 2006 replacing the De La Salle University System.

The institution started as an exclusive all-boys elementary and high school. In 1920, it began offering a two-year Associate in Arts Commerce program, which was later discontinued in 1931 in favor of a Bachelor of Science in Commerce program. The patron saint of the university is St. John Baptist de La Salle, the Vatican's patron saint for those who work in education.

De La Salle University has been cited by the Philippine Commission on Higher Education (CHED) as a Center of Excellence in 14 of its programs, and a Center of Development in 5. The university is among 40 institutions granted autonomous status by CHED as of 2010. It is the first of only two institutions granted the highest-level accreditation (Level IV) by the Philippine Accrediting Association of Schools, Colleges and Universities (PAASCU). The university is a member of the ASEAN University Network (AUN) and International Association of Universities (IAU) as well as the local South Manila Inter-Institutional Consortium.